LOCUS       40924_23619       11447 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB665 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Gateway Binary Vectors with Organelle-Targeted Fluorescent Proteins 
            for Highly Sensitive Reporter Assay in Gene Expression Analysis of 
            Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11447)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane 
            University, Interdisciplinary Center for Science Research; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11447)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11447)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane University, 
            Interdisciplinary Center for Science Research; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2071..2091
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2092..2808
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      2833..3085
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3119..3135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3143..3159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3167..3197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3212..3233)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3269..3521)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3684..4232)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4284..4463)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4969..4993)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5514..6302
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6551..7139
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7325..7465)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7809..8003)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8072..9142)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9574..10200)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggct ccaaagaaga agagaaaggt catggtgagc
     2101 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2161 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2221 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2281 accttcacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2341 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2401 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2461 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2521 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2581 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2641 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2701 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2761 ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaagta agcttagagc
     2821 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     2881 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     2941 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     3001 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     3061 gcggtgtcat ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatggt
     3121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3181 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa
     3241 ttaggcgcgc ctatttctat taattcccga tctagtaaca tagatgacac cgcgcgcgat
     3301 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     3361 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     3421 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     3481 ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt cgagctgggg
     3541 acgtccccag ctcggtaccc cctcctgggc acggtcgtac gcgatccgcc gcggttccac
     3601 ctcaccattg cgggtatgcc agttgagttc gatgagttcc cgtaccagct ccaactcggg
     3661 cgatgccacg cgcactccgt tcagatctcg gtgacgggca ggaccggacg gggcggtacc
     3721 ggcaggctga agtccagctg ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg
     3781 gccgcccgca gcatgccgcg gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg
     3841 tcgttgggca gcccgatgac agcgaccacg ctcttgaagc cctgtgcctc cagggacttc
     3901 agcaggtggg tgtagagcgt ggagcccagt cccgtccgct ggtggcgggg ggagacgtac
     3961 acggtcgact cggccgtcca gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg
     4021 atgccggcga cctcgccgtc cacctcggcg acgagccagg gatagcgctc ccgcagacgg
     4081 acgaggtcgt ccgtccactc ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt
     4141 gtctcgatgt agtggttgac gatggtgcag accgccggca tgtccgcctc ggtggcacgg
     4201 cggatgtcgg ccgggcgtcg ttctgggctc atatcttatt gccccccggg atcagatctg
     4261 gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg
     4321 gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc
     4381 gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag
     4441 tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca
     4501 tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcttga tcccctgcgc
     4561 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     4621 ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct
     4681 agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc
     4741 cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg
     4801 gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt
     4861 gaagctgatc cgtcgagatt aatagaaata aattcagcct aattcggcgt taattcagta
     4921 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     4981 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     5041 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     5101 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     5161 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     5221 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     5281 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     5341 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     5401 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     5461 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     5521 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     5581 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     5641 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     5701 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     5761 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     5821 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     5881 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     5941 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     6001 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     6061 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     6121 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     6181 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     6241 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     6301 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     6361 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     6421 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     6481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6661 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7201 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7261 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7321 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     7381 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     7441 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     7501 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     7561 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     7621 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     7681 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     7741 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     7801 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     7861 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     7921 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     7981 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     8041 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     8101 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     8161 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     8221 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     8281 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     8341 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     8401 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     8461 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     8521 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     8581 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     8641 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     8701 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     8761 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     8821 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     8881 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     8941 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     9001 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
     9061 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
     9121 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
     9181 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
     9241 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
     9301 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
     9361 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
     9421 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
     9481 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
     9541 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
     9601 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
     9661 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
     9721 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
     9781 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
     9841 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
     9901 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
     9961 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
    10021 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
    10081 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
    10141 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
    10201 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
    10261 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
    10321 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
    10381 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
    10441 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
    10501 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
    10561 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
    10621 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
    10681 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
    10741 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
    10801 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
    10861 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    10921 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    10981 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    11041 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    11101 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    11161 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    11221 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    11281 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    11341 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
    11401 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
